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Simple and sensitive assay of dopamine beta-hydroxylase in human cerebrospinal fluid
Summary
Type:
HPLC
Application #:
102010
Catalog#:
720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection:
electrochemical: potential 0.9V versus Ag/AgCl
Conditions:
eluent: 0.05M K phosphate buffer pH 7.2 containing 20% methanol,
flow rate 1.0ml/min
Authors:
Suzuki H
Source:
J Chromatogr 341 (1985) 176 - 181
Details
Legend: Chromatograms using DBH incubation mixture of human child CSF as enzyme. (A) Fusaric acid blank incubation, (B) experimental incubation, (C) 50pmol octopamine added to the fusaric acid blank;
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